LABEL-FREE CELL SORTING

Sort Smarter.

Not Harder.

Introducing the World’s First Label-Free Cell Separation Technology.

Elegantly simple workflow.

LevitasBio has introduced the first platform that sorts cells without any markers or modifications.  For the first time, researchers have access to all high-value primary samples regardless of starting cell number or viability.  Our first product, the LeviCell, delivers simple, label-free cell separation in a fast, reliable and low-maintenance solution to sort cell populations.  Thus, the LeviCell enables studies and beneficial uses of previously inaccessible, high-value primary samples and sensitive cell types.

Levitas LeviCell Gen 1.0

1. Sample introduction

Sample pipetted into Levitas Consumable.

Actual view of levitated cells.

2. Automated Label-Free Sorting

Magnetic force levitation based on cell density/characteristics.

3. Separation & Sorting

Levitated cells transition into separate collection ports for later removal.

How It Works​

Levitas technology separates individual cells according to type or state based on the different levitation heights caused by their unique magnetic and density signatures.

Why Levitate?​

Our LeviCell system presents a fast, simple, and highly-flexible platform requiring minimal training and no calibration.

Higher Sorting Accuracy

  • Separate populations down to individual cells
  • Cells are gently collected in an unaltered state
  • No negative impact on population representation

High sample purity

  • No dyes or labels are used
  • No cell stress or pressure
  • No gene expression changes in primary cells

Faster workflow

  • Various cell types can be imaged, counted, and classified within a single workflow
  • Debris removal included in the process
  • RBC removal included in the process

Ease of use

  • Run to run reproducibility without re-calibration
  • Sterility: Fits within a cell culture hood or biosafety cabinet
  • No debris removal, pre-purification, or aerosols required

Flexibility

  • Works with large cells and clusters including:
    Embryos, Animals, Organoids
  • Cells can be suspended in any cell media or liquid

High Yield and Viability

  • Rare cell sensitivity – limit of detection
  • Ability to culture post enrichment
  • Able to sort with very low cell count

Steps and time required to operate.

Levitas LeviCell radically cuts the number of steps and total time to effectively collect targeted cells.

Levitas technology can also be used to separate cells based on real-time response to drugs and stressors without the need to culture or label the cells.

The platform is simple, allowing users to begin with unprocessed whole blood samples or any sample with cells in suspension.
In a matter of minutes, the instrument and its software automatically perform levitation; label-free separation; quantification of results based on high-resolution imaging; and analysis by cell number, morphology, and levitation profile.
In a matter of minutes, the instrument and its software automatically perform levitation; label-free separation; quantification of results based on high-resolution imaging; and analysis by cell number, morphology, and levitation profile.

Application areas we are exploring​

Sample Prep​

Especially useful with precious or difficult samples, magnetic levitation allows users to separate live from dead cells in a fast, simple and gentle process.

Bacteriology​

Real-time drug response offers a fast, accurate, and simple approach to bacterial detection and antibiotic resistance testing.

Oncology​

For detection and screening of circulating tumor cells, the Levitas system solves key technical challenges.

Stem Cells​

Effective, fast, and simple separation of differentiated cells from stem cells and iPSC, allows a culture of homogeneous cell populations.

Immunology​

Accurate separation and identification of key cell types, such as activated leukocytes, could overcome key challenges in CAR-T therapy.

What would you Levitate?​

We are always curious to learn about potential application areas for our technology. If you have ideas for research and collaboration, let us know!